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RESEARCH PAPER
Control of infection of Biomphalaria alexandrina (Ehrenberg, 1831) with Schistosoma mansoni Sambon, 1907 using Eucalyptus camaldulensis
1
Medical Malacology Laboratory, Theodor Bilharz Research Institute, Giza, Egypt
2
Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
Submission date: 2018-04-04
Final revision date: 2018-05-30
Acceptance date: 2018-06-06
Publication date: 2018-07-25
Corresponding author
Mohamed R. Habib
Medical Malacology Laboratory, Theodor Bilharz Research Institute, 12411 Giza, Egypt
Medical Malacology Laboratory, Theodor Bilharz Research Institute, 12411 Giza, Egypt
Folia Malacol. 2018;26(3):155–165
KEYWORDS
ethyl acetate extractEucalyptus camaldulensisBiomphalaria alexandrinaSchistosoma mansoniOxidative stressantioxidant activity
ABSTRACT
Schistosomiasis infection can be interrupted if the development of miracidia to cercariae in Biomphalaria alexandrina (Ehrenberg) is blocked. This requires an efficient snail’s antioxidant system which can be complemented with an exogenous antioxidant source to alleviate the oxidative stress of infection. For this purpose, leaves of Eucalyptus camaldulensis were air-dried, extracted with aqueous methanol (85%) and defatted with petroleum ether. The obtained defatted extract was used to prepare extracts with different solvents. Among these, ethyl acetate showed the highest antioxidant activity and was chosen for the experiments. The effect of ethyl acetate extract on the infection, survivorship, as well as levels of malondialdehyde (MDA), catalase (CAT) and reduced glutathione (GSH) in B. alexandrina were measured in control, untreated infected and treated infected snails on 1, 10, and 30 days post infection (dpi). The snails exposed to ethyl acetate extract showed a significant reduction in the infection rate compared to those infected and untreated. Moreover, the extract decreased the level of MDA and increased CAT and GSH activities in the haemolymph and tissues of the treated snails. The results suggest that ethyl acetate extract from leaves of E. camaldulensis can be used as an antiparasitic compound against the intramolluscan phase of S. mansoni Sambon, 1907.
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CITATIONS (4):
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Phytochemical analysis of Eucalyptus camaldulensis leaves extracts and testing its antimicrobial and schistosomicidal activities
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