Identification of gender-associated mitochondrial haplotypes in Anodonta anatina (Bivalvia: Unionidae)
More details
Hide details
Department of Genetics, University of Szczecin, Poland
Submission date: 2007-12-01
Acceptance date: 2008-02-03
Publication date: 2020-06-09
Corresponding author
Marianna Soroka   

Department of Genetics, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland
Folia Malacol. 2008;16(1)
Doubly uniparental inheritance (DUI) of mitochondrial DNA, different from maternal inheritance, is known to occur in five bivalve families (Mytilidae, Veneridae, Unionidae, Margaritiferidae, Hyriidae). DUI involves two types of mitochondrial DNA: F type, inherited from the mother and M type, inherited from the father. Females have only F type mtDNA, males have both types, M type being located in their gonads, F type in their somatic tissues. Among freshwater bivalves known to show DUI, only Anodonta woodiana occurs in Poland. The aim of this study was to ascertain if DUI occurred in another native bivalve, Anodonta anatina, based on DNA sequence of mitochondrial gene of cytochrome oxidase subunit I (cox1). M haplotype was found in male gonads, and F haplotype in somatic tissues of both sexes. Seven sequences were obtained for each F and M haplotypes, 625–709 base pairs long. Variation of 0.2 and 0.3% was found within F andMcox1 sequences, respectively, and 29–32% variation between them.
Araujo R., Gomez I., Machordom A. 2005. The identity and biology of Unio mancus Lamarck, 1819 (=U. elongatulus) (Bivalvia: Unionidae) in the Iberian Peninsula. J. Moll. Stud. 71: 25-31.
Breton S., Beaupre H. D., Stewart D. T., Hoeh W. R., Blier P. U. 2007. The unusual system of doubly uniparental inheritance of mtDNA: isn't one enough? Trends Genet. 23: 465-474.
Burzyński A., Zbawicka M., Skibinski D. O. F., Wenne R. 2003. Evidence for recombination of mtDNA in the marine mussel Mytilus trossulus from the Baltic. Mol. Biol. Evol. 20: 388-392.
Curole J. P., Kocher T. D. 2002. Ancient sex-specific extension of the cytochrome c oxidase II gene in Bivalves and the fidelity of Double-Uniparental Inheritance. Mol. Biol. Evol. 19: 323-1328.
Dalziel A. C., Stewart D. T. 2002. Tissue-specific expression of male-transmitted mitochondrial DNA and its implications for rates of molecular evolution in Mytilus mussels (Bivalvia: Mytilidae). Genome 45: 348-355.
Folmer O., Black M., Hoeh W., Lutz R., Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 3: 294-299.
Garrido-Ramos M. A., Stewart B., Southerland B., Zouros E. 1998. The distribution of male-transmitted and female-transmitted mitochondrial DNA types in somatic tissues of blue mussels: implication for the operation of doubly uniparental inheritance of mitochondrial DNA. Genome 41: 818-824.
Giribet G., Wheeler W. 2002. On bivalve phylogeny: a high-level analysis of the Bivalvia (Mollusca) based on combined morphology and DNA sequence data. Invertebr. Biol. 121: 271-324.
Graf D. L., Ó Foighil D. 2000. The evolution of brooding characters among the freshwater pearly mussels (Bivalvia: Unionoidea) of North America. J. Moll. Stud. 66: 157-170.
Hoeh W. R., Stewart D. T., Sutherland B. W., Zouros E. 1996. Multiple origins of gender-associated mitochondrial DNA lineages in bivalves (Mollusca: Bivalvia). Evolution 50: 2276-2286.
Hoeh W. R., Stewart D. T., Saavedra C., Sutherland B. W., Zouros E. 1997. Phylogenetic evidence for role-reversals of gender-associated mitochondrial DNA in Mytilus (Bivalvia: Mytilidae). Mol. Biol. Evol. 14: 959-967.
Hoeh W. R., Stewart D. T., Guttman S. I. 2002. High fidelity of mitochondrial genome transmission under the doubly uniparental model of inheritance in freshwater mussels (Bivalvia: Unionoidea). Evolution 56: 2252-2261.
Källersjö M., Von Proschwitz T., Lundberg S., Eeldenäs P., Erséus CH. 2005. Evaluation of ITS rDNA as a complement to mitochondrial gene sequences for phylogenetic studies in freshwater mussels: an example using Unionidae from north-western Europe. Zool. Scr. 34: 415-424.
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120.
Lee T., Ó Foighil D. 2004. Hidden Floridian biodiversity: mitochondrial and nuclear gene trees reveal four cryptic species within the scorched mussel, Brachidontes exustus, species complex. Mol. Ecol. 13: 3527-3542.
Liu H-P., Mitton J. B., Herrmann S. J. 1996a. Genetic differentiation in and managment recommendations for the freshwater mussel, Pyganodon grandis (Say, 1829). Am. Malacol. Bull. 13: 117-124.
Liu H-P., Mitton J. B., Wu S-K. 1996b. Paternal mitochondrial DNA differentiation far exceeds maternal mitochondrial DNA and allozyme differentiation in the freshwater mussel, Anodonta grandis grandis. Evolution 50: 952-957.
Mock K. E., Brim-Box J. C., Miller M. P., Downing M. E., Hoeh W. R. 2004. Genetic diversity and divergence among freshwater mussel (Anodonta) populations in the Bonneville Basin of Utah. Mol. Ecol. 13: 1085-1098.
Passamonti M., Scali V. 2001. Gender-associated mitochondrial DNA heteroplasmy in the venerid clam Tapes philippinarum (Mollusca Bivalvia). Curr. Genet. 39: 117-124.
Passamonti M., Boore J. L., Scali V. 2003. Molecular evolution and recombination in gender-associated mitochondrial DNAs of the manila clam Tapes philippinarum. Genetics 164: 603-611.
Quesada H., Wenne R., Skibinski D. O. F. 1999. Interspecies transfer of female mitochondrial DNA is coupled with role-reversal and departure from neutrality in the mussel Mytilus trossulus. Mol. Biol. Evol. 16: 655-665.
Skibinski D. O. F., Gallagher C., Beynon C. M. 1994a. Sex-limited mitochondrial DNA in the marine mussel Mytilus edulis. Genetics 138: 801-809.
Skibinski D. O. F., Gallagher C., Beynon C. M. 1994b. Mitochondrial-DNA inheritance. Nature 368: 817-818.
Soroka M. 2005. Genetic variability among freshwater mussel Anodonta woodiana (Lea, 1834) (Bivalvia: Unionidae) populations recently introduced in Poland. Zool. Sci. 22: 1137-1144.
Soroka M. 2008. Doubly uniparental inheritance of mitochondrial DNA in freshwater bivalve Anodonta woodiana (Bivalvia: Unionidae). Folia Biol. 56: 91-95.
Soroka M., Grygieńczo-Raźniewska E. 2005. Mitochondrial DNA-based diagnostic molecular markers for freshwater bivalves. Folia Malacol. 13: 145-152.
Stepien C. A., Hubers A. N., Skidmore J. L. 2002. Diagnostic genetic markers and evolutionary relationships among invasive dreissenoid and corbiculoid bivalves in North America: phylogenetic signal from mitochondrial 16S rDNA. Mol. Phylogenet. Evol. 13: 31-49.
Therriault T. W., Docker M. F., Orlova M. I., Heath D. D., Macisaac H. J. 2004. Molecular resolution of the family Dreissenidae (Mollusca: Bivalvia) with emphasis on Ponto-Caspian species, including first report of Mytilopsis leucophaeata in the Black Sea basin. Mol. Phylogenet. Evol. 30: 479-489.
Walker J. M., Curole, J. P., Wade D. E., Chapman E. G., Bogan A. E., Watters G. T., Hoeh W. R. 2006. Taxonomic distribution and phylogenetic utility of gender- associated mitochondrial genomes in the Unionoida (Bivalvia). Malacologia 48: 265-282.
Zbawicka M., Skibinski D. O. F., Wenne R. 2003. Doubly uniparental transmission of mitochondria DNA length variants in the mussel Mytilus trossulus. Marine Biology 142: 455-460.
Zouros E., Ball A. O., Saavedra C., Freeman K. R. 1994a. An unusual type of mitochondrial DNA inheritance in the blue mussel Mytilus. Proc. Natl. Acad. Sci. USA 91: 7463-7467.
Zouros E., Ball A. O., Saavedra C., Freeman K. R. 1994b. Mitochondrial-DNA inheritance. Nature 368: 818.
Journals System - logo
Scroll to top