A new species of Bythinella Moquin-Tandon, 1856 (Gastropoda: Caenogastropoda: Bythinellidae) from south-western France
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Département de Malacologie, Musée d’Histoire naturelle et d’Ethnographie de Colmar, Colmar, France
Malacologie, Etude et Conservation des Mollusques continentaux, France
Jean-Michel Bichain   

Département de Malacologie, Musée d’Histoire naturelle et d’Ethnographie de Colmar, 11 rue Turenne, 68000, Colmar, France
Submission date: 2022-04-04
Final revision date: 2022-07-27
Acceptance date: 2022-08-03
Online publication date: 2022-09-02
Publication date: 2022-09-02
Folia Malacol. 2022;30(3):143–154
The spring-snail genus Bythinella is a diverse group of minute operculate gastropods, usually restricted to springs and widely distributed in Europe. Species are primarily delineated based on shell characters such as size or overall shape. Nevertheless, studies based on molecular and morphological approaches show that these characters can be highly variable and do not necessarily have taxonomic significance. In this paper we formally describe and name, according to the requirements of the International Code of Zoological Nomenclature, a species identified by previously published phylogenetic analyses and by a barcoding gap approach based on the mitochondrial COI gene. The molecular results suggest this species is a micro-endemic species currently recognized only from south-western France at its type locality. We also highlight that the barcode approach considerably reduces the number of valid species in this region, and that a strictly shell-based taxonomy probably overestimates the species richness of the genus Bythinella.
Benke M., Brändle M., Albrecht C., Wilke T. 2009. Pleistocene phylogeography and phylogenetic concordance in cold-adapted spring snails (Bythinella spp.). Molecular Ecology 18: 890–903.
Benke M., Brändle M., Albrecht C., Wilke T. 2011. Patterns of freshwater biodiversity in Europe: lessons from the spring snail genus Bythinella. Journal of Biogeography 38: 2021–2032.
Bernasconi R. 2000. Révision du genre Bythinella (Moquin-Tandon, 1855) (Gastropoda, Prosobranchia, Hydrobiidae, Amnicolinae, Bythinellini). Documents Malacologiques Hors-série n°1: 1–126.
Bichain J.-M. 2007. Apports des outils moléculaires et morphométriques à la délimitation des espèces du genre Bythinella Moquin-Tandon, 1856 (Mollusca, Caenogastropoda, Amnicolidae). Muséum National d’Histoire Naturelle, Paris, France.
Bichain J.-M. 2010. La systématique des bythinelles revisitée. Editions Universitaires Européennes, Sarrebruck.
Bichain J.-M., Boisselier-Dubayle M.-C., Bouchet P., Samadi S. 2007a. Species delimitation in the genus Bythinella (Mollusca: Caenogastropoda: Rissooidea): A first attempt combining molecular and morphometrical data. Malacologia 49: 293–311.
Bichain J.-M., Boudsocq C., Prié V. 2004. Les mollusques souterrains du réseau karstique de Padirac (Lot, France) et micro-répartition de Bythinella padiraci Locard, 1903 (Mollusca, Caenogastropoda, Rissooidea). Karstologia 43: 9–18.
Bichain J.-M., Gaubert P., Samadi S., Boisselier-Dubayle M.-C. 2007b. A gleam in the dark: Phylogenetic species delimitation in the confusing spring-snail genus Bythinella Moquin-Tandon, 1856 (Gastropoda: Rissooidea: Amnicolidae). Molecular Phylogenetics and Evolution 45: 927–941.
Boeters H. D. 1973. Die Gattung Bythinella und Gattung Marstoniopsis in Westeuropa, 1. Westeuropäische Hydrobiidae, 4 (Prosobranchia). Malacologia 14: 271–285.
Boeters H. D. 1979. Species concept of prosobranch freshwater molluscs in Western Europe. Malacologia 18: 57–60.
Boeters H. D. 1981. Die Gattung Bythinella Moquin-Tandon in Deutschland (Prosobranchia). Archiv für Molluskenkunde 111: 191–205.
Boeters H. D. 1992. On the invasion of subterranean waters by small prosobranchs in Central Europe. Abstract of the 11th World Congress of Malacology, Siena (Italy), 30 August – 5 September 1992: 331–333.
Boeters H. D. 1998. Mollusca: Gastropoda: Superfamilie Rissooidea, Süsswasserfauna von Mitteleuropa. Gustav Fischer Verlag, Jena – Lübeck Ulm.
Bofill A., Haas F., Aguilar-Amat J. D. de 1921. Estudi sobre la malacologia de les valls pirenaiques VI. Estudi sobre la fauna malacològica de les conques del besòs, ter, fluvià, muga i litorals intermitjes. Treballs del Museu de Ciències Naturals de Barcelona 3: 832–1241.
Bole J., Velkovrh F. 1986. Mollusca from continental subterranean aquatic habitats. In: Botosaneanu L. (ed.). Stygofauna mundi: a faunistic, distributional, and ecological synthesis of the world fauna inhabiting subterranean waters (including the marine interstitial). E.J. Brill/W. Backhuys, Leiden, pp. 177–208.
Boubée N. 1833. Nouveaux gisemens, en France, de Mollusques et Zoophytes. Bulletin d’Histoire Naturelle de France 1(3): 3–24. https://biodiversitylibrary.or....
Cox L. R. 1960. Thoughts on the classification of the Gastropoda. Proceedings of the Malacological Society of London 33: 239−261.
Cuvier G. 1795. Second Mémoire sur l’organisation et les rapports des animaux à sang blanc, dans lequel on traite de la structure des Mollusques et de leur division en ordre, lu à la société d’Histoire Naturelle de Paris, le 11 prairial an troisième [30 May 1795]. Magazin Encyclopédique, ou Journal des Sciences, des Lettres et des Arts, 1795 [1. année] 2: 433–449. https://www.biodiversitylibrar....
Davis G. M. 1993. Evolution of prosobranch snails transmitting Asian Schistosoma; coevolution with Schistosoma: A review. In: Sun T. (ed.). Progress in Clinical Parasitology. Springer, New York, NY, pp. 145–204.
Des Moulins C. 1827. Catalogue des espèces et variétés de Mollusques testacés terrestres et fluviatiles observés jusqu’à ce jour, à l’état vivant, dans le département de la Gironde et dans l’arrondissement subsidiaire de la Société Linnéenne de Bordeaux. Bulletin d’Histoire naturelle de la Société linnéenne de Bordeaux 2: 39–69. https://www.biodiversitylibrar....
Drouët H. 1867. Mollusques terrestres et fluviatiles de la Côte-d’Or. Baillière et fils, Paris. https://www.biodiversitylibrar....
Dupuy D. 1849. Catalogus extramarinorum Galliae Testaceorum in opere cui titulus ‘Histoire naturelle des Mollusques terrestres et d’eau douce qui vivent en France’ descriptorum. 4 pp.
Dupuy D. 1851. Histoire naturelle des mollusques terrestres et d’eau douce qui vivent en France. Cinquième fascicule: 1 pp. (title page), 459–594, pl. 22–24 (May 1851). Victor Masson/Brun, Paris. https://biodiversitylibrary.or....
Falniowski A. 1987. Hydrobioidea of Poland (Proso­branchia: Gastropoda). Folia Malacologica 1: 1–122.
Falniowski A., Hofman S., Rysiewska A. 2016. A new species of Bythinella Moquin-Tandon, 1855 (Caenogastropoda: Truncatelloidea) from Naxos Island, Greece. Folia Malacologica 24: 185–192.
Falniowski A., Horsák M., Szarowska M. 2009. Bythinella hansboetersi Glöer et Pešić, 2006 (Gastropoda: Rissooidea) in Bulgaria: its morphology, molecular distinctness, and phylogeography. Folia Malacologica 17: 11–20.
Falniowski A., Szarowska M., Fiałkowski W., Mazan K. 1998. Unusual geographic pattern of interpopulation variation in a spring snail Bythinella (Gastropoda: Prosobranchia). Journal of Natural History 32: 605–616.
Fedosov A., Achaz G., Gontchar A., Puillandre N. 2022. Mold, a novel software to compile accurate and reliable DNA diagnoses for taxonomic descriptions. Molecular Ecology Resources 22: 2038–2053.
Frauenfeld G. von 1857. Über die Paludinen aus der Gruppe der Pal. viridis Poir. Sitzungsberichte der Kaiserlichen Akademie der Wissenschaften, Mathematisch-Naturwissenschaftliche Classe 22(2): 569–578, 1 pl. [«1856»]. https://www.biodiversitylibrar....
Gittenberger E. 1991. What about non-adaptive radiation? Biological Journal of the Linnean Society 43 (4): 263–272.
Gittenberger E. 2004. Radiation and adaptation, evolutionary biology and semantics. Organisms Diversity & Evolution 4: 135–136.
Giusti F., Pezzoli E. 1977. Primo contributo alla revisione del genere Bythinella in Italia. Natura Bresciana, Annali del Museo Civico di Storia Naturale di Brescia 14: 3–66.
Giusti F., Pezzoli E. 1982. Notes on the small Hydrobioidea in Italian subterranean waters: catalogue, biogeography and some systematic problems. Malacologia 22: 463–468.
Glöer P. 2022. The freshwater gastropods of the West-Palaearctis. Volume 2: Moitessieriidae, Bythinellidae, Stenothyridae. Gloër, Hetlingen.
Haase M., Wilke T., Mildner P. 2007. Identifying species of Bythinella (Caenogastropoda: Rissooidea): A plea for an integrative approach. Zootaxa 1563: 1–16.
Hebert P. D. N., Cywinska A., Ball S. L., DeWaard J. R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B: Biological Sciences 270(1512): 313–321.
Hlaváč J. C. 2002. Molluscan fauna of the Javorıcsky Karst (Czech Republic, central Moravia). Malacological Newsletter 20: 93–105.
ICZN (International Commission on Zoological Nomenclature) 2012. Amendment of Articles 8, 9, 10, 21 and 78 of the International Code of Zoological Nomenclature to expand and refine methods of publication. ZooKeys 219: 1–10.
INPN 2022. Inventaire national du patrimoine naturel (INPN). Available online at (accessesed 18 February 2022).
Kadolsky D. 2005. Case 3321. Bythinella Moquin-Tandon, 1856 (Mollusca, Gastropoda, Prosobranchia, Rissooidea): proposed conservation of usage by the designation of Bulimus viridis Poiret, 1801 as the type species. Bulletin of Zoological Nomenclature 62: 134–138.
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120.
Kumar S., Stecher G., Li M., Knyaz C., Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35: 1547–1549.
Locard A. 1884. Description d’une espèce nouvelle de mollusque gastéropode. Bulletin de la Société des Amis des Sciences Naturelles de Rouen 20: 433–436.
Locard A. 1893. Conchyliologie française. [I]. Les coquilles des eaux douces et saumâtres de France. Description des familles, genres et espèces. J.-B. Baillière, Paris.
Locard A. 1903. Description de mollusques nouveaux appartenant à la faune souterraine de France et d’Italie. Bulletin du Muséum National d’Histoire Naturelle 8: 607–610.
Mazan K. 2000. Morphological and allozymic variation within and between populations of Bythinella Moquin-Tandon, 1855 (Gastropoda: Prosobranchia), I. Morphological characters. Folia Malacologica 8: 107–139.
Mazan K., Szarowska M. 2000a. Morphological and allozymic variation within and between populations of Bythinella Moquin-Tandon, 1855 (Gastropoda: Prosobranchia), II. Phenetic analysis. Folia Malacologica 8: 189–213.
Mazan K., Szarowska M. 2000b. Morphological and allozymic variation within and between populations of Bythinella Moquin-Tandon, 1855 (Gastropoda: Prosobranchia), III. Phylogenetic analysis. Folia Malacologica 8: 257–269.
MolluscaBase 2022. Molluscabase. Available online at (accessed 18 February 2022).
Moquin-Tandon A. 1855–1856. Histoire naturelle des mollusques terrestres et fluviatiles de France, Tome premier (1): i–viii, 1–14 + Atlas: 1–16, pl. 1–9 (12 April 1855); (2): 145–256 + Atlas: 17–32, pl. 10–18 (4 May 1855); (3): 257–416 + Atlas: 33–48, pl. 19–27 (1 August 1855). Tome second (4): i–iii, 1–176 + Atlas: 49–64, pl. 28–36 (10 September 1855); (5): 177–368 + Atlas: 65–80, pl. 37–45 (2 January 1856); (6): 369–646 + Atlas: i–iii, 81–92, pl. 46–54 (9 April 1856). J.-B. Baillière, Paris. https://biodiversitylibrary.or....
Paladilhe A. 1867. Nouvelles miscellanées malacologiques, IV. Espèces inédites, nouvelles ou peu connues du département de l’Hérault. Nouvelles miscellanées malacologiques 2e fascicule: 39–53, pl. 2, figs 10–21, pl. 3, figs 23–30. F. Savy, Paris. https://www.biodiversitylibrar....
Paladilhe A. 1870. Prodrome à l’histoire malacologique de la France. Étude monographique sur les Paludinidées françaises. Annales de Malacologie 1(2): 167–224; 1(3): 225–244, Paris. https://biodiversitylibrary.or....
Paladilhe A. 1874. Monographie du nouveau genre Peringia suivie de descriptions d’espèces nouvelles de Paludinidées françaises. Annales des Sciences Naturelles (Zoologie et Paléontologie) ser. 6, 1(2): 1–38, pl. 3. Paris. https://www.biodiversitylibrar....
Poiret J. L. M. 1801. Coquilles fluviatiles et terrestres observées dans le Département de l’Aisne et aux environs de Paris: prodrome. Barrois et Soissons, Paris. xi + 119 pp. https://www.biodiversitylibrar....
Prié V., Bichain J.-M. 2009. Phylogenetic relationships and description of a new stygobite species of Bythinella (Mollusca, Gastropoda, Caenogastropoda, Amnicolidae) from southern France. Zoosystema 31: 987–1000.
Prié V., Cucherat X. 2021. Additional molecular data on the protected springsnail species Bythinella viridis (Poiret, 1801) (Gastropoda: Bythinellidae) suggest synonymy of related taxa. Knowledge & Management of Aquatic Ecosystems 422: 36 (1–7).
Puillandre N., Brouillet S., Achaz G. 2021. ASAP: assemble species by automatic partitioning. Molecular Ecology Resources 21: 609–620.
Ratnasingham S., Hebert P. D. N. 2007. Barcoding, BOLD: The Barcode of Life Data System ( Molecular Ecology Notes 7: 355–364.
Rohlf F. J. 2001. TpsDig Program version 1.23. Stony Brook, NY: Department of Ecology and Evolution, State University of New York.
Rundell R. J., Price T. D. 2009. Adaptive radiation, nonadaptive radiation, ecological speciation and nonecological speciation. Trends in Ecology & Evolution 24: 394–399.
Samadi S., Barberousse A. 2006. The tree, the network and the species. Biological Journal of the Linnean Society 89: 509–521.
Statsoft 2001. Statistica version 6.
Szarowska M., Osikowski A., Hofman S., Falniowski A. 2016. Do diversity patterns of the spring-inhabiting snail Bythinella (Gastropoda, Bythinellidae) on the Aegean Islands reflect geological history? Hydrobiologia 765: 225–243.
Szarowska M., Wilke T. 2004. Sadleriana pannonica (Frauenfeld, 1865): a lithoglyphid, hydrobiid or amnicolid taxon? Journal Molluscan Studies 70: 49–57.
Thompson J. D., Higgins D. G., Gibson T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673–4680.
UICN Comité Français, OFB & MNHN 2021. La liste rouge des espèces menacées en France. Chapitre Mollusques continentaux de France métropolitaine. Paris, France.
Wilke T., Benke M., Brändle M., Albrecht C., Bichain J.-M. 2010. The neglected side of the coin: non-adaptive radiations in spring snails (Bythinella spp.). In: Glaubrecht M. (ed.). Evolution in action. Springer, Berlin, Heidelberg, pp. 551–578.