This study expands and supplements the knowledge of aquatic molluscs of selected public greenhouses, especially in zoological and botanical gardens, in the Czech Republic. In total, 14 freshwater species were found at 23 sites situated mostly in botanical and zoological gardens. Freshwater molluscs were divided into four groups: species occurring in the Czech Republic only in greenhouses, non-native species living also in the wild, native species and alien genotypes of native species. The most frequent mollusc of greenhouses was Physella acuta (Draparnaud). Melanoides tuberculata (O. F. Müller) and Helisoma cf. duryi (Wetherby) of the first group were other very common and widespread greenhouse species. On the other hand Marisa cornuarietis (Linnaeus), Bithynia tentaculata (Linnaeus), Lymnaea stagnalis (Linnaeus), Gyraulus crista (Linnaeus) and Ferrissia californica (Rowell) were found at only one studied site.