RAPD markers as a tool for analysis of relationships among selected species of Lymnaeidae (Gastropoda: Pulmonata)
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Department of Cell Biology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
Andrzej Lesicki   

Department of Cell Biology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
Submission date: 2007-12-01
Acceptance date: 2008-02-03
Publication date: 2020-06-09
Folia Malacol. 2008;16(1):39–51
Total DNA samples, isolated from several dozen individuals identified on the basis of morphological and anatomical features as Lymnaea stagnalis (L.), Stagnicola corvus (Gmelin), S. palustris (O. F. Müller), S. turricula (Held), and S. occulta (Jackiewicz) were used for amplification of DNA fragments by RAPD. In this way, molecular characters of each individual were identified. Pairwise distances were the smallest (0.0008–0.0188) among conspecific individuals from the same population. Conspecifics from different populations were generally less similar (pairwise distances 0.0177–0.0589). Greater differences were observed among individuals of different species (distances 0.1257–0.6505, i.e. at least 10-fold larger than distances within and between populations). RAPD markers are useful for analyses of taxonomic relationships within the Lymnaeidae. The results confirm the specific status of L. stagnalis, S. corvus and S. occulta. A separate group is formed by S. palustris/turricula, although these two taxa are very similar, and RAPD analysis does not explain if they are distinct species or subspecies.
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