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RESEARCH PAPER
RAPD markers as a tool for analysis of relationships among selected species of Lymnaeidae (Gastropoda: Pulmonata)
1
Department of Cell Biology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
2
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
Submission date: 2007-12-01
Acceptance date: 2008-02-03
Publication date: 2020-06-09
Corresponding author
Andrzej Lesicki
Department of Cell Biology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
Department of Cell Biology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznań, Poland
Folia Malacol. 2008;16(1):39–51
KEYWORDS
ABSTRACT
Total DNA samples, isolated from several dozen individuals identified on the basis of morphological and anatomical features as Lymnaea stagnalis (L.), Stagnicola corvus (Gmelin), S. palustris (O. F. Müller), S. turricula (Held), and S. occulta (Jackiewicz) were used for amplification of DNA fragments by RAPD. In this way, molecular characters of each individual were identified. Pairwise distances were the smallest (0.0008–0.0188) among conspecific individuals from the same population. Conspecifics from different populations were generally less similar (pairwise distances 0.0177–0.0589). Greater differences were observed among individuals of different species (distances 0.1257–0.6505, i.e. at least 10-fold larger than distances within and between populations). RAPD markers are useful for analyses of taxonomic relationships within the Lymnaeidae. The results confirm the specific status of L. stagnalis, S. corvus and S. occulta. A separate group is formed by S. palustris/turricula, although these two taxa are very similar, and RAPD analysis does not explain if they are distinct species or subspecies.
REFERENCES (36)
1.
Armbruster G. 1997. Evaluations of RAPD markers and allozyme patterns - evidence for morphological convergence in the morphotype of Cochlicopa lubricella (Gastropoda, Pulmonata, Cochlicopidae). J. Moll. Stud. 63: 379-388. https://doi.org/10.1093/mollus....
2.
Backeljau T., Bruyn L., Wolf H., Jordanes K., Dongen S., Verhagen R., Winnepenninckx B. 1995. Random amplified polymorphic DNA (RAPD) and parsimony methods. Cladistics 11: 119-130. https://doi.org/10.1111/j.1096....
3.
Backeljau T., Bruyn L., Wolf H., Jordanes K., Dongen S., Verhagen R., Winnepenninckx B. 1996. Multiple UPGMA and Neighbor-joining Trees and the performance of some computer packages. Mol. Biol. Evol. 13: 309-313. https://doi.org/10.1093/oxford....
4.
Bargues M. D., Vigo M., Horak P., Dvorak J., Patzner R. A, Pointier J. P., Jackiewicz M., Meier-Brook C., Mas-Coma S. 2001. European Lymnaeidae (Mollusca: Gastropoda), intermediate hosts of trematodiases, based on nuclear ribosomal DNA ITS-2 sequences. Infection, Genetics and Evolution 1: 85-107. https://doi.org/10.1016/S1567-....
5.
Bargues M. D., Horak P., Patzner R. A, Pointier J. P., Jackiewicz M., Meier-Brook C., Mas-Coma S. 2003. Insights into the relationships of Palearctic and Nearctic lymnaeids (Mollusca: Gastropoda) by rDNA ITS-2 sequencing and phylogeny of stagnicoline intermediate host species of Fasciola hepatica. Parasite 10: 243-255. https://doi.org/10.1051/parasi....
6.
Benzie J. A. H., Williams S. T. 1992. Genetic structure of giant clam (Tridacna maxima) populations from reefs in the Western Coral Sea. Coral Reefs 11: 135-141. https://doi.org/10.1007/BF0025....
7.
Caetano-Anolles G. 1994. MAAP: a versatile and universal tool for genome analysis. Plant Mol. Biol. 25: 1011-1026. https://doi.org/10.1007/BF0001....
8.
Crossland S., Coates D., Grahame J., Mill P. J. 1993. Use of random amplified polymorphic DNAs (RAPDs) in separating two sibling species of Littorina. Mar. Ecol. Prog. Ser. 96: 301-305. https://doi.org/10.3354/meps09....
9.
Falkner G. 1995. Present-day knowledge on the systematics of the genus Stagnicola in Europe. Abst. 12th Intern. Malacol. Congr., Vigo, Spain.
10.
Falkner G., Bank R. A., Proschwitz T. von 2001. Check-list of the non-marine molluscan species-group taxa of states of northern Atlantic and central Europe (CLECOM I). Heldia 4: 1-76.
11.
Felsenstein J. 1995. PHYLIP (Phylogeny Inference package) v. 3.57c. University of Washington.
12.
Fiedorow P., Szweykowska-Kulińska Z. 1997. The influence of polyamines on polymerase chain reaction (PCR). Acta Biochim. Polon. 44: 83-88. https://doi.org/10.18388/abp.1....
13.
Hosaka K., Hanneman J. R. E. 1994. Random amplified polymorphic DNA markers detected in a segregating hybrid population of Solanum chacoense × S. phureja. Jpn. J. Genet. 69: 53-66. https://doi.org/10.1266/jjg.69....
14.
Jackiewicz M. 1959. Badania nad zmiennością i stanowiskiem systematycznym Galba palustris (O. F. Müll.). Pr. Kom. biol. Pozn. TPN 19: 89-187.
15.
Jackiewicz M. 1993. Phylogeny and relationships within the European species of the family Lymnaeidae (Gastropoda: Pulmonata: Basommatophora). Folia Malacol. 5: 61-95. https://doi.org/10.12657/folma....
16.
Jackiewicz M. 1998. European species of the family Lymnaeidae (Gastropoda, Basommatophora: Pulmonata). Genus 9: 1-93.
17.
Jackiewicz M. 2000. Błotniarki Europy (Gastropoda: Pulmonata: Lymnaeidae). Wydawnictwo Kontekst, Poznań.
18.
Jacobsen R., Forbes V. E., Skovgaard O. 1996. Genetic population structure of the prosobranch snail Potamopyrgus antipodarum (Gray) using PCR-RAPD fingerprints. Proc. Biol. Sci. 263: 1065-1070. https://doi.org/10.1098/rspb.1....
19.
Jones C. S., Noble L. R., Ouma J., Kariuki H. C., Mimpfoundi R., Brown D. S., Rollinson D. 1999. Molecular identification of schistosome intermediate hosts: case studies of Bulinus forskalii group species (Gastropoda: Planorbidae) from central and east Africa. Biol. J. Linn. Soc. 68: 215-240. https://doi.org/10.1006/bijl.1....
20.
Kilias R. 1992. Vergleichend-anatomische Untersuchungen an "Galba palustris (O. F. Müller)" von ungarischen Fundorten als beitrag zur palustris-Problematik (Gastropoda, Basommatophora: Lymnaeidae). Malak. Abh. 16: 25-29.
21.
Klein-Lankhorst R. M., Vermunt A., Weide R., Liharska T., Zabel P. 1991. Isolation of molecular markers for tomato (L. esculentum) using random amplified polymorphic DNA (RAPD). Theor. Appl. Genet. 83: 108-114. https://doi.org/10.1007/BF0022....
22.
Klinbunga S., Ampayup P., Tassanakajon A., Jarayabhand P., Yoosukh W. 2001. Genetic diversity and molecular markers of cupped oysters (Genera Crassostrea, Saccostrea, and Striostrea) in Thailand revealed by Randomly Amplified Polymorphic DNA analysis. Mar. Biotech. 3: 133-144. https://doi.org/10.1007/s10126....
23.
Langand J., Barral V., Delay B., Jourdane J. 1993. Detection of genetic diversity within snail intermediate host of the genus Bulinus by using random amplified polymorphic DNA markers (RAPDs). Acta Tropica 55: 205-215. https://doi.org/10.1016/0001-7....
24.
Meier-Brook C., Bargues M. D. 2002. Catascopia, a new genus for three nearctic and one palearctic Stagnicola species (Gastropoda, Lymnaeidae). Folia Malacol. 10: 83-84. https://doi.org/10.12657/folma....
25.
Nei M., Li W.-H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA 76: 5269-5273. https://doi.org/10.1073/pnas.7....
26.
Pacak A., Fiedorow P., Dabert J., Szweykowska-Kulińska Z. 1998. RAPD technique for taxonomic studies of Pellia epiphylla - complex (Hepaticae, Metzgeriales). Genetica 104: 179-187. https://doi.org/10.1023/A:1003....
27.
Primrose S. B. 1999. Zasady analizy genomu. Przewodnik do mapowania i sekwencjonowania DNA różnych organizmów. Wydawnictwa Naukowo-Techniczne, Warszawa.
28.
Rybska E., Pacak A., Szweykowska-Kulińska Z., Lesicki A. 2000. Taxonomy of European Lymnaeidae (Gastropoda: Pulmonata) in studies with the use of molecular biology techniques. I. Preliminary view on the subgenus Stagnicola Leach, 1830 on the basis of RAPD analysis. Folia Malacol. 8: 277-284. https://doi.org/10.12657/folma....
29.
Schmidt H., Westheide W. 1999. Genetic relationships (RAPD-PCR) between geographically separated populations of the "cosmopolitan" interstitial polychaete Hesionides gohari (Hesionidae) and the evolutionary origin of the freshwater species Hesionides riegerorum. Biol. Bull. 196: 216-226. https://doi.org/10.2307/154256....
30.
Stothard J. R., Mgeni A. F., Alawi K. S., Salvioli L., Rollinson D. 1997. Observation on shell morphology, enzymes and random amplified polymorphic DNA (RAPD) in Bulinus africanus group snails (Gastropoda: Planorbidae) in Zanzibar. J. Moll. Stud. 63: 489-503. https://doi.org/10.1093/mollus....
31.
Stothard J. R., Rollinson D. 1996. An evaluation of Random Amplified Polymorphic DNA (RAPD) for the identification and phylogeny of freshwater snails of the genus Bulinus (Gastropoda: Planorbidae). J. Moll. Stud. 62: 165-176. https://doi.org/10.1093/mollus....
32.
Stothard J. R., Rollinson D. 1997. Partial DNA sequences from the mitochondrial cytochrome oxidase subunit I (COI) gene can differentiate the intermediate hosts Bulinus globosus and B. nasutus (Gastropoda: Planorbidae). J. Nat. Hist. 31: 727-737. https://doi.org/10.1080/002229....
33.
Taiwu L., Wenxin Y., Xiurong S., Zhibiao Y., Hao G. 2004. RAPD analysis of genetic diversities of three species of abalone. 23: 1139-1142.
34.
Throp J. P. 1982. The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Am. Rev. Ecol. Syst. 13: 139-168. https://doi.org/10.1146/annure....
35.
Webster J. P., Davies C. M., Ndamba J., Noble L.R., Jones C. S., Woolhouse M. E. J. 2001. Spatio-temporal genetic variability in the schistosome intermediate host Biomphalaria pfeifferi. Ann. Trop. Med. Parasit. 95: 515-527. https://doi.org/10.1080/000349....
36.
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res. 18: 6531-6535. https://doi.org/10.1093/nar/18....
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