Effects of temperature and photoperiod on glucose, glycerol and glycogen concentrations in Helix pomatia Linnaeus, 1758 in spring and autumn
More details
Hide details
Department of Animal Physiology, Institute of General and Molecular Biology, N. Copernicus University, Toruń, Poland
Anna Nowakowska   

Department of Animal Physiology, Institute of General and Molecular Biology, N. Copernicus University, 87-100 Toruń, Poland
Submission date: 2010-12-28
Acceptance date: 2011-02-28
Publication date: 2011-10-10
Folia Malacol. 2011;19(3):155–163
One of the variables oscillating seasonally in Helix pomatia L., described in our previous paper, was their rudimentary cryoprotection provided by modest increases in haemolymph glucose and glycerol concentrations in autumn and early spring, respectively. Because factors governing cryoprotective responses are unknown, we decided to investigate the effects of spring and autumn acclimation of H. pomatia to different thermal and photoperiod conditions on the changes in haemolymph concentrations of glucose and glycerol and on the glycogen level in selected organs. Neither acclimation to short-day photoperiod nor low ambient temperatureevoked increase in glucose and glycerol concentration in spring and autumn. Both acclimation variants decreased hepatopancreatic glycogen level. The rudimentary freeze-tolerance in H. pomatia seems to be acombined effect of cold and short-day photoperiod and might also be affected by their nutritional and reproductive status. The effect of exposure to frost-bite is also likely to be involved.
Aarset A. V. 1982. Freezing tolerance in intertidal invertebrates (a review). Comp. Biochem. Physiol. 73A: 571-580.
Ansart A., Vernon P. 2004. Cold hardiness abilities vary with the size of the land snail Cornu aspersum. Comp. Biochem. Physiol. 139A: 205-211.
Ansart A., Vernon P., Charrier M., Daguzan J. 2002. The effect of antibiotic treatment on the supercooling ability of the land snail Helix aspersa (Gastropoda: Pulmonata). Cryobiology 44: 189-192.
Biannic M., Coillot J. P., Daguzan J. 1994. Heart rate in relation to dehydration in the snail Helix aspersa Müller (Gastropoda, Pulmonata). Comp. Biochem. Physiol. 108A: 65-67.
Biannic M., Daguzan J. 1993. Cold-hardiness and freezing in the land snail Helix aspersa Müller (Gastropoda; Pulmonata). Comp. Biochem. Physiol. 104A: 503-506.
Bielawski J., Kęsa H. 1986. Seasonal variation of phosphatase activity in the hepatopancreas of the snail Helix pomatia L. Comp. Biochem. Physiol. 83A: 105-107.
Borges E., Vuaden F. C., Cognato G. D. P., Fauth M. D. G., Bonan C. D., Turcato G., Rossi I. C. D. E., Dias R. D. 2004. Effects of starvation on haemolymphatic glucose levels, glycogen contents and nucleotidase activities in different tissues of Helix aspersa (Müller, 1774) (Mollusca, Gastropoda). J. Exp. Zool. 301A: 891-897.
Bride J., Bonnefoy-Claudet R., Gomot L. 1993. Effect of temperature on haemolymphatic glucose and albumen gland polysaccharides during dormancy in the snail Helix aspersa maxima. Comp. Biochem. Physiol. 106A: 701-705.
Caputa M., Nowakowska A., Rogalska J., Wentowska K. 2005. Winter torpor in Helix pomatia: regulated defence mechanism or forced inactivity? Can. J. Zool. 83: 1608-1613.
Grenot C. J., Garcin L., Dao J., Hérold J. P., Fahys B., Tséré-Pagés H. 2000. How does the European common lizard, Lacerta vivipara, survive the cold of winter? Comp. Biochem. Physiol. 127A: 71-80.
Han E. N., Bauce E. 1995. Glycerol synthesis by diapausing larvae in response to the timing of low temperature exposure, and implications for overwintering survival of the spruce budworm, Choristoneura fumiferana. J. Insect Physiol. 41: 981-985.
Layne J. R. Jr 1995. Seasonal variation in the cryobiology of Rana sylvatica from Pennsylvania. J. Therm. Biol. 20: 349-353.
Li Y. P., Ding L., Goto M. 2002. Seasonal changes in glycerol content and enzyme activities in overwintering larvae of the Shonai ecotype of the rice stem borer, Chilo suppressalis Walker. Arch. Insect Biochem. Physiol. 50: 53-61.
Lazaridou-Dimitriadou M., Saunders D. S. 1986. The influence of humidity, photoperiod, and temperature on the dormancy and activity of Helix lucorum L. (Gastropoda. Pulmonata). J. Moll. Stud. 52: 180-189.
Michaelidis B., Kyriakopoulou-Sklavounou P., Staikou A., Papathanasiou I., Konstantinou K. 2008. Glycolytic adjustments in tissues of frog Rana ridibunda and land snail Helix lucorum during seasonal hibernation. Comp. Biochem. Physiol. 151A: 582-589.
Nicolai A., Vernon P., Lee M., Ansart A., Charrier M. 2005. Supercooling ability in two populations of the land snail Helix pomatia (Gastropoda: Helicidae) and ice-nucleating activity of gut bacteria. Cryobiology 50: 48-57.
Nowakowska A., Caputa M., Rogalska J. 2006. Seasonal changes in cryoprotectants concentrations in Helix pomatia snails. J. Physiol. Pharmacol. 57: 93-105.
Riddle W. A. 1981. Cold hardiness in the woodland snail, Anguispira alternata (Say) (Endodontidae). J. Therm. Biol. 6: 117-120.
Seifter S., Dayton S., Novic B., Muntwyler E. 1950. The estimation of glycogen with the anthrone reagent. Arch. Biochem. 25: 191-200.
Steiner A. A., Petenusci S. O., Brentegani L. G., Branco L. G. S. 2000. The importance of glucose for the freezing tolerance/intolerance of the anuran amphibians Rana catesbeiana and Bufo paracnemis. Rev. Brasil. Biol. 60: 321-328.
Storey J. M., Storey K. B. 1985. Adaptations of metabolism for freeze tolerance in the gray tree frog, Hyla versicolor. Can. J. Zool. 63: 49-54.
Storey K. B. 1987. What contributes to freeze tolerance? NIPS 2: 157-160.
Storey K. B. 2006. Reptile freeze tolerance: Metabolism and gene expression. Cryobiology 52: 1-16.
Storey K. B., Baust J. G., Storey J. M. 1981. Intermediary metabolism during low temperature acclimation in the overwintering gall fly larva, Eurosta solidaginis. J. Comp. Physiol. 144B: 183-190.
Storey K. B., Storey J. M. 1988. Freeze tolerance in animals. Physiol. Rev. 68: 27-84.
Voituron Y., Hérold J. P., Grenot C. 2000. Metabolic adaptations of overwintering European common lizard (Lacerta vivipara). Physiol. Biochem. Zool. 73: 264-270.
Voituron Y., Storey J. M., Grenot C., Storey K. B. 2002. Freezing survival, body ice content and blood composition of the freeze-tolerant European common lizard, Lacerta vivipara. J. Comp. Physiol. 172B: 71-76.
Watanabe M., Tanaka K. 1999. Cold tolerance strategy of the freeze-intolerant chrysomelid, Aulacophora nigripennis (Coleoptera: Chrysomelidae), in warm-temperate regions. Eur. J. Entomol. 96: 175-181.
The Hepato-Hypothalamic-Pituitary-Adrenal-Renal Axis: Mathematical Modeling of Cortisol’s Production, Metabolism, and Seasonal Variation
Kamau Pierre, Naomi Schlesinger, Ioannis Androulakis
Journal of Biological Rhythms
Physiological and biochemical responses to cold and drought in the rock-dwelling pulmonate snail, Chondrina avenacea
Vladimír Koštál, Jan Rozsypal, Pavel Pech, Helena Zahradníčková, Petr Šimek
Journal of Comparative Physiology B